The youngster, who was last year diagnosed with both acute lymphoblastic leukaemia and acute myeloid leukaemia, has become known around the country.
A short video posted on the 'Team Margot' Facebook page shows the youngster, from Essington, repeating her father Yaser's words: 'just one more'.
That mimics a campaign run by blood cancer charity and bone marrow register Anthony Nolan.
A caption on the video says: "Margot encourages those on the stem cell register to encourage 'Just One More' to do the same."
Earlier this week Margot's parents revealed they would meet doctors to 'discuss their options' after finding out news the levels of leukaemia in their daughter's system have increased.
The family revealed the news on their Facebook page to more than 16,000 supporters of the youngster's campaign.
The 'Swab4Margot' campaign, backed by celebrities such as Stephen Fry and John Bishop, led to more than 50,000 people requesting swab kits from Delete Blood Cancer UK.
Eventually a match was discovered but last month father Yaser and mother Vicki revealed their daughter had suffered a relapse. The family's hope it was a slight set-back were dashed last week when they revealed the disease had progressed from a one per cent presence to somewhere between 60 and 80 per cent.
Yaser said: "Margot appears well – she runs around doing all the things that two-year-old girls do, and she looks healthy.
"In that sense, we can hardly believe that she's got this disease in her. If a doctor was to tell us that actually, they'd made a terrible mistake and she was actually fine, we'd instantly believe them.
"Our focus now is just to hang on, enjoy every day we spend together and to help others going through the same thing.
"Campaigning for a donor partly helped us feel like we were actually doing something to help."