Jota has joined the Premier League champions in a deal worth up to £45million, while Matt Doherty left earlier this summer for Tottenham, for £15m.
Wolves, meanwhile, have snapped up young defender Ki-Jana Hoever from the Reds for an initial £9m.
And when asked if Wolves are becoming a stepping stone to the proverbial big six, Nuno said: "This is not my view.
"Jota is a player who has been with us for three seasons with such high performances.
"We're not a stepping stone. Players that join us really want to play and progress with us, not looking for the next move.
"That is definitely not our idea, definitely not."
Nuno believes Wolves are a place where top players can fulfil their ambitions, as he said: "Wolves is a huge club. A big, big club.
"We are a big, big club, and we are proud to say that."
Fresh face Hoever is considered a top prospect, but Nuno believes the Dutchman can make an impact in the side sooner rather than later.
"Not only as a prospect, he can also give solutions (now) to us as he's a versatile player who can go inside our shape," said the Wolves chief.
"He can do two clear positions, so this is the profile of player we want.
"I'm looking forward to helping Ki become better and he'll be a very good option for us."
On Jota's move to Anfield, he added: "It's a fantastic club with a fantastic manager that I believe can take the best out of Diogo."