Despite being on the bench for Saturday’s draw with Blackpool, Chambers took part in just one light train session last week after injuring his hamstring.
Ismail meanwhile missed the game against the Tangerines through illness. But Whitney says both are available tonight.
“Everyone trained yesterday so it’s looking good from that point of view,” the boss said.
“Zeli Ismail was ill for the Blackpool game but he’s back in the mix for the MK Dons game.
“Adam Chambers trained for 15 minutes on Friday so it would have been a risk to play him against Blackpool.
“But he came through training yesterday and looks good.
“It has been all positive in training. We’ve been very proactive in looking at how to cause MK Dons problems.
“We’ll look to remain solid but we won’t be going there to park the bus. We’re going there to win.”
Robbie Neilson’s Dons enter tonight’s game having fallen to back-to-back defeats against Bradford and Portsmouth.
And Whitney’s has warned his team to be wary of a backlash.
“We’re approaching a tough side,” the boss continued.
“When you go there, you know they have got pace in wide positions and key men all across the pitch.
“We’ve got to respect them. They’ll be hurting a little bit after the Portsmouth defeat and they’ll be looking for a reaction.
“We have got to be ready for an MK Dons team on the top of their game - but we’re going there confident that we can cause them problems.
“We’ve got to take our performance levels on now. We have drawn a lot of games so far and it’s a fine line between those draws into wins.”
Meanwhile, Nielson has pinpointed Erhun Oztumer as the Saddlers main threat.
“Walsall’s front three are very good,” he said.
“They’ve got good pace and Oztumer as a 10 is very dangerous.
“We have to be wary of him, not to give him and space or free kicks around the box.
“If we go out and play as well as we can, we will be expecting to pick up full points.”