Eyez Barberz, based on Staveley Road, Whitmore Reans, was found to be operating – and carrying out mobile hairdressing services in the area.
Officers from Wolverhampton Council and West Midlands Police have now served a prohibition notice – shutting them down.
Council cabinet member Councillor Steve Evans,said: “The council take a zero-tolerance approach to those businesses who are seen to not be adhering to the rules, and who are presenting a serious risk to members of the public during the pandemic.
“The Governments rules and regulations are clear, and those offering the services of both barbering and hairdressing should remain closed until further notice.
“We urge members of the public and other businesses to report any concerns to the environmental health and trading standards team."
The closure came after evidence was gathered by the authority's environmental health and trading standards team – along with West Midlands Police.
Government chiefs have already ordered all non-essential businesses and public spaces to be closed due to coronavirus.
The action comes after Wolverhampton pub The Pendulum, Blaydon Road, Pendeford, had its premises license suspended on Friday for opening behind closed doors.
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