The Portuguese forward, 22, joined up with the rest of the squad for Friday's session following a hamstring injury.
He picked up the injury in the 2-0 win over Bournemouth at Molineux on December 15 and has not played a part in the four games since.
On the condition of Jota, who netted 17 times as Wolves took the Championship by storm last term, boss Nuno Espirito Santo said: "He has started training.
"The moment he starts training with the team, he's always an option for us."
Wolves' other concern was midfielder Romain Saiss, who went off with a knee knock in Wednesday's 2-0 defeat to Crystal Palace.
But he has trained without any problems too.
"Everybody has trained, so everybody is fit," added Nuno.