Barriers have been erected by officials and the route tested by competitors today, just one day before the races which will see extensive road closures.
There will be two races on Thursday, with the Women's Individual Time Trial starting at 10am and the Men's Individual Time Trial following at 12 noon.
The route spans cross West Park, Goldthorn Park, Sedgley, Dudley town centre, Gornal and Himley.
West Park in Wolverhampton will be the show-piece venue for the start and finish of the event, as well as the medal ceremonies, with a sold-out crowd ready to cheer the competitors as they get underway.
An exclusive time-lapse video showing the full Black Country cycling time trial can be viewed here.
Councillor Patrick Harley, leader elect of Dudley Council, said: “This is a significant event through the heart of Dudley and gives us an opportunity to showcase what we have in the borough on an international stage.
"This is our opportunity to be part of a global event and I am delighted we have been chosen to do that.
"It will of course naturally cause some disruption to roads while it is on, so I would urge people to check out the list of road closures and plan their journeys accordingly.”