Lioness Kayli was one of the oldest members of the West Midland Safari Park's pride, located in the carnivore section of the safari drive-through.
She was the mother of Charlie, Amber and Ambush, who were born at the park in 2012, and also a grandmother to Amber's four cubs, who were born in 2019.
Kayli passed away at the age of 16.
West Midland Safari Park took to social media to announce Kayli's death and pay tribute to her.
A spokesman said: "It’s with great sadness and heavy hearts that we share the news that female lion Kayli has passed away at the age of 16.
"She was one of the oldest in our pride and mum to Charlie, Amber and Ambush, who also reside at the park after being born here back in 2012.
"Kayli's legacy will also live on as she became a grandmother to Amber's four cubs, born in summer 2019.
"She will be dearly missed by keepers who have worked closely with her over the years."
Hundreds of people offered their condolences on social media, sharing photos and memories of visiting Kayli over the years.