Emergency services were called to the serious property fire in Regent Road, in the Handsworth area, at around 11.10pm on Tuesday.
The incident involved a severe fire involving the entire three-storey property, including the roof. The second floor of the property has been destroyed by the fire.
A man and a woman, both believed to be in their 20s, who were inside the building at the time, suffered potentially serious injuries from escaping the building. West Midlands Ambulance Service (WMAS) say they were unharmed from the fire itself.
West Midlands Fire Service has now launched an investigation into the cause of the blaze.
A fire service spokesman said: "Fire investigators from West Midlands Fire Service are continuing their work at the scene of a severe house fire this morning in Regent Road, Handsworth. WMFS responded to the incident at 11.11pm on Tuesday, January 26.
"The first fire crew arrived in under two minutes. At the height of the incident, WMFS resources at the scene included six fire engines and a hydraulic platform, crewed by around 35 firefighters.
"This was a severe fire involving the entire three-storey, semi-detached property, including the roof. The second floor was destroyed by fire, virtually all of the first floor and part of the ground floor.
"A woman and a man, who are believed to have been injured when they jumped to safety from a first-floor window, were treated by West Midlands Ambulance Service.
"Firefighters used hose reel jets and main jets to bring the fire under control. They faced challenging conditions, the fire having made much of the structure and what remained of the roof unstable.
"We worked closely with several agencies at the scene, including the ambulance service, police and utility companies.
"At around 2am our resources were scaled down to three fire engines and a hydraulic platform, with the final crew leaving the scene shortly before 9am."
A spokesman for WMAS said: "When crews arrived, they were presented with two patients who had been inside the building at the time of the fire, a man and woman.
“Although unharmed from the fire itself, the patients had both suffered potentially serious injuries from escaping the building.
“They were treated at the scene for their injuries before being taken to Queen Elizabeth Hospital in separate ambulances for further treatment.”
Three ambulances, three paramedic officers, the Hazardous Area Response Team (HART) and the MERIT trauma car with a doctor and critical care paramedic onboard attended the scene.
A West Midlands Police spokesman added: "We were alerted to a fire at a house in Regent Road, Handsworth Wood, just after 11pm yesterday [Tuesday].
"On arrival officers found two people, a man and a woman both in their 20s, being treated by paramedics.
"They were taken to hospital with serious injuries but neither are believed to be life-threatening.
"At this stage we are working in partnership with West Midlands Fire Service to ensure that the building is safe and to establish the cause of the fire."