The shop, called New Wellness Zone, was reopened on last Tuesday where the Tipton Twins, who are both aged 96, cut the ribbon.
However less than 10 days later, the store - which is run by Kerry Clarke, granddaughter of Lilian and great niece of Doris - will close due to the second lockdown.
Ms Clarke, aged 35 from Hagley, called this "really bad timing" for her business.
She said: "It’s really bad timing for us. Our hairdressing manager started this Monday with her team of four due to start shortly.
"We are opened from Monday to Wednesday this week and we will relaunch ready to open for December.
"We have a great team and can’t wait for people to visit us. We will focus on reopening and open as soon as the government says it’s safe to do so.
"We are definitely looking forward to 2021 and putting 2020 behind us."
Ms Clarke renovated the shop, based at Crystal Hose, on Enterprise Drive, in Stourbridge, during the first lockdown and also re-branded the name.
Asked the special appearance of her grandmother and great aunt, she continued: "Lil and Doris came to have their hair done as VIP guests last month, with only them in the salon. We gave them each a Kérastase goody bag.
"It was the first time they had their hair permed in over six months due to lockdown. They were delighted with the service and level of treatment received.
"We were planning on having a big grand opening but unfortunately with everything going on, we had to re-plan and keep it low key but invited them back to Wellness Zone to cut the ribbon, to officially open the place.
"They did a bit of Yoga and also had their nails done - matching colours of course."
Lilian and Doris, also known as The Tipton Twins, have become celebrities over the past year where they have appeared on TV including ITV's Good Morning Britain show.
Customers can access yoga, hair and beauty treatments, health treatments and a spa all under one roof. They can also get free parking directly outside.
The store has created 10 new jobs and 10 self-employed yoga teachers work for the shop, said Ms Clarke.
The store is run by a family business which also operates other companies in the region including Soccer Zone, in Halesowen, Boing Zone, in Stourbidge, and Inflatable Zone, in Halesowen.
During lockdown, the centre will be offering online yoga classes, the delivery of spa and hair care packages which are available from its website.
For more information, visit www.wellness-zone.co.uk.