The late film star's 1934 Indian Sport Scout bike featured among the collection on offer at the Bonhams Stafford Sale held yesterday at Staffordshire County Showground.
The bike was valued between £55,000 and £65,000 and eventually sold for £59,740.
McQueen was a known motorcycle enthusiast and it is not the first time one of his former models has been sold in Stafford.
James Stansel, from Bonhams, said McQueen's cars and motorcycles were the envy of many a collector and Indian was believed to be his favoured marque.
He said the 1934 Indian 750cc Sport Scout was one of Indian's most successful models of the 1930s.
The sale took place at The Classic Motorcycle Mechanics Show.