Staff organise the trail each year over half term for children and their families to enjoy.
Some of the 150-acre site's trees are given faces and personalities, and this year have been given various outfits to help bring them to life.
They also each have a biography explaining their character, such as Lucinda Pearl-Montgomery, who is chair of the local WI, who loves to opera and a chance to wear her 'fir' coat.
Denise Brownridge, who works at the arboretum, said: "This year we thought we would make it different by adding clothing to the faces, they all have different characters.
"Saturday was busy and it was the first day of the trail.
"The warm, sunny weather definitely helped, it's the first time people have been able to come outside without the risk of getting wet for a few weeks.
"It was lovely to see families out enjoying themselves.
"We have tried to include a pun on trees or forests into each bio to make it quite funny, I actually think adults will like it as well as we have injected a bit of humour into things."
The walk takes around 20 minutes, but admission includes access to the rest of the arboretum which has more than five miles of walks to enjoy.
Denise added: "We have some beautiful walks that people don't realise were there.
"One of the things we are trying to do with these trails is to introduce people to all the different walks they can take, we also have a gorgeous laburnum arch which is now in flower.
"The walk for the Tree People Trail takes guests around the poplar trees, it's nice to encourage people to explore all the different areas."
The trail runs until June 6.